miR-194-5p靶向调控BCKDHA基因的表达

doi:10.11843/j.issn.0366-6964.2017.08.005

Abstract

Abstract:

This study aimed to predict and validate the crucial miRNA regulating BCKDHA gene expression, where BCKDHA was α subunit of BCKDH (Branched-chain α-ketoacid dehydrogenase complex), which was a key rate-limiting enzyme in the catabolism of branched-chain amino acids. Three online softwares were used to predict miRNAs targeting BCKDHA. QPCR was used to detect expressions of the crucial miRNA and BCKDHA mRNA in 3T3-L1 cells during its differentiation and after over-expressing the crucial miRNA, respectively. The results showed that miR-194-5p was a key miRNA regulating BCKDHA expression with fully complementation between its seed sequence and the 190-196th bases of BCKDHA 3'UTR. The expression of miR-194-5p decreased while that of BCKDHA mRNA increased with differentiation. The target relationship was also validated by dual luciferase reporter results which showed that miR-194-5p negatively regulated the expression of BCKDHA gene. The expression of BCKDHA mRNA was significantly reduced(P<0.05) after over-expressing miR-194-5p. It is concluded that miR-194-5p inhibits the expression of BCKDHA by targeting its 3' UTR.

CLC Number:

S813.1

ZHANG Ying-jiao, QIAO Li-ying, JING Jiong-jie, PAN Yang-yang, MA Yuan, GUO Yun-li, WEI Qiong, LIU Wen-zhong. miR-194-5p Targets BCKDHA Gene to Regulate Its Expression[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1416-1423.

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miR-194-5p Targets BCKDHA Gene to Regulate Its Expression

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